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The Recombinant Full-length Human Cellular tumor antigen p53 (TP53) is a full-length protein produced in yeast. This recombinant TP53 protein contains 393 amino acids and is tagged N-terminally with a 6xHis-tag. It has a calculated molecular weight of 45.7kDa, with purity greater than 90% as determined by SDS-PAGE. Based on its functions, this TP53 protein may be used in the studies of its gene-specific transcription regulation with intracellular delivery of the protein per se and cancers. TP53, generally called p53, is a nuclear transcription factor involved in the induction of cell cycle arrest or apoptosis by transactivating a large number of target genes. Functionally active p53 normalizes the cell cycle through completely repairing damaged DNA. Once serious DNA damage is irreversible, pro-apoptotic p53 clears these cells with severe DNA lesions thus preventing the transfer of damaged DNA to daughter cells. Therefore, p53 also functions to sustain genomic integrity.
The Recombinant Full-length Human Cellular tumor antigen p53 (TP53) is a full-length protein produced in yeast. This recombinant TP53 protein contains 393 amino acids and is tagged N-terminally with a 6xHis-tag. It has a calculated molecular weight of 45.7kDa, with purity greater than 90% as determined by SDS-PAGE. Based on its functions, this TP53 protein may be used in the studies of its gene-specific transcription regulation with intracellular delivery of the protein per se and cancers.
TP53, generally called p53, is a nuclear transcription factor involved in the induction of cell cycle arrest or apoptosis by transactivating a large number of target genes. Functionally active p53 normalizes the cell cycle through completely repairing damaged DNA. Once serious DNA damage is irreversible, pro-apoptotic p53 clears these cells with severe DNA lesions thus preventing the transfer of damaged DNA to daughter cells. Therefore, p53 also functions to sustain genomic integrity.
| Cat.No | ACP04729 | Target Name | TP53 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 1-393aa | Mol Weight | 45.7kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P04637 |
|---|
Uniprot Id
P04637
Target Species
Human
Target Name
TP53
Target Full Name
Cellular tumor antigen p53
Target Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Target Involvement
Esophageal cancer (ESCR); Li-Fraumeni syndrome (LFS); Squamous cell carcinoma of the head and neck (HNSCC); Lung cancer (LNCR); Papilloma of choroid plexus (CPP); Adrenocortical carcinoma (ADCC); Basal cell carcinoma 7 (BCC7)
Target Subcellular Location
Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.; [Isoform 1]: Nucleus. Cytoplasm. Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; [Isoform 2]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 3]: Nucleus. Cytoplasm. Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells.; [Isoform 4]: Nucleus. Cytoplasm. Note=Predominantly nuclear but translocates to the cytoplasm following cell stress.; [Isoform 7]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 8]: Nucleus. Cytoplasm. Note=Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.; [Isoform 9]: Cytoplasm.
Target Protein Families
P53 family
Target Tissue Specificity
Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3
Target Research Area
Cell Biology, Apoptosis
Target Synonyms
Antigen NY-CO-13; BCC7; Cellular tumor antigen p53; FLJ92943; LFS1; Mutant tumor protein 53; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; tumor antigen p55; Tumor protein 53; Tumor protein p53; Tumor suppressor p53
Target Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
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