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The process for producing recombinant Human CHMP7 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human CHMP7 protein (1-453aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human CHMP7 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
The process for producing recombinant Human CHMP7 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human CHMP7 protein (1-453aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human CHMP7 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
| Cat.No | ACP01950 | Target Name | CHMP7 |
|---|---|---|---|
| Target Synonyms | Chromatin-modifying protein 7 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-453aa |
| Mol Weight | 56.9 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8WUX9 |
|---|
Uniprot Id
Q8WUX9
Target Species
Human
Target Name
CHMP7
Target Full Name
Charged multivesicular body protein 7
Target Function
ESCRT-III-like protein required to recruit the ESCRT-III complex to the nuclear envelope during late anaphase. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway.
Target Subcellular Location
Cytoplasm. Nucleus envelope.
Target Protein Families
SNF7 family
Target Research Area
Signal Transduction
Target Synonyms
CHMP7Charged multivesicular body protein 7; Chromatin-modifying protein 7
Target Background
Involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. Located in cytosol; nuclear envelope; and nucleoplasm. Part of ESCRT III complex. Colocalizes with chromatin.
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