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The preparation of this recombinant Human CKMT1A protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a CKMT1A fragment (40-417aa) that could be translated into the CKMT1A protein and then transferred it into E.coli cells to express the recombinant CKMT1A protein molecule. In order to get the target protein with high purity, N-terminal 6xHis tag was used in the production. The purity is 90% determined by SDS-PAGE.Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Studies suggested that mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation. In addition, CKMT1 is a key regulator of the permeability transition pore through a complex that is distinct from the classical permeability transition pore. The knock-down of the mitochondrial creatine kinase-1 by RNA interference causes the dissipation of the mitochondrial membrane potential. Mitochondrial creatine kinase-1 has been found to serve as a universal and functionally necessary gatekeeper of the PT-pore, as its depletion induces mitochondrial depolarization and apoptotic cell death.
The preparation of this recombinant Human CKMT1A protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a CKMT1A fragment (40-417aa) that could be translated into the CKMT1A protein and then transferred it into E.coli cells to express the recombinant CKMT1A protein molecule. In order to get the target protein with high purity, N-terminal 6xHis tag was used in the production. The purity is 90% determined by SDS-PAGE.Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Studies suggested that mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation. In addition, CKMT1 is a key regulator of the permeability transition pore through a complex that is distinct from the classical permeability transition pore. The knock-down of the mitochondrial creatine kinase-1 by RNA interference causes the dissipation of the mitochondrial membrane potential. Mitochondrial creatine kinase-1 has been found to serve as a universal and functionally necessary gatekeeper of the PT-pore, as its depletion induces mitochondrial depolarization and apoptotic cell death.
| Cat.No | ACP02891 | Target Name | CKMT1A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 40-417aa | Mol Weight | 47.1kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P12532 |
|---|
Uniprot Id
P12532
Target Species
Human
Target Name
CKMT1A
Target Full Name
Creatine kinase U-type, mitochondrial
Target Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Target Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Target Protein Families
ATP:guanido phosphotransferase family
Target Research Area
Signal Transduction
Target Synonyms
Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; CKMT1B; Creatine kinase mitochondrial 1 (ubiquitous); Creatine kinase mitochondrial 1B; Creatine kinase U type mitochondrial; Creatine kinase U-type; KCRU_HUMAN; Mia-CK; mitochondrial; U-MtCK; Ubiquitous mitochondrial creatine kinase; UMTCK
Target Background
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.
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