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To produce recombinant Human POLQ protein, a well-established recombinant DNA technology is the key. A DNA template of POLQ was constructed with N-terminal 10xHis tag & C-terminal Myc tag using the technique. Once the template was made, the recombinant Human POLQ protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 321-554aa of the Human POLQ. The purity of this recombinant is 85% determined by SDS-PAGE. POLQ?(POLH)?is a protein coding gene that encodes DNA polymerase theta. According to some studies, POLQ may have the following features.POLQ inhibits genomic instability and genomic rearrangements derived from DNA double-strand breaks. POLQ is a member of a similar cluster of up-regulated genes that are strongly associated with poor clinical outcomes in breast, ovarian and other types of cancer. Inhibition of POLQ is a compelling combination therapy for radiosensitization. POLQ-deficient mutants displayed hypersensitivity to H2O2-induced oxidative alkali damage, but not to UV light or cisplatin. Overexpression of POLQ leads to poor prognosis in patients with early breast cancer.
To produce recombinant Human POLQ protein, a well-established recombinant DNA technology is the key. A DNA template of POLQ was constructed with N-terminal 10xHis tag & C-terminal Myc tag using the technique. Once the template was made, the recombinant Human POLQ protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 321-554aa of the Human POLQ. The purity of this recombinant is 85% determined by SDS-PAGE. POLQ?(POLH)?is a protein coding gene that encodes DNA polymerase theta. According to some studies, POLQ may have the following features.POLQ inhibits genomic instability and genomic rearrangements derived from DNA double-strand breaks. POLQ is a member of a similar cluster of up-regulated genes that are strongly associated with poor clinical outcomes in breast, ovarian and other types of cancer. Inhibition of POLQ is a compelling combination therapy for radiosensitization. POLQ-deficient mutants displayed hypersensitivity to H2O2-induced oxidative alkali damage, but not to UV light or cisplatin. Overexpression of POLQ leads to poor prognosis in patients with early breast cancer.
| Cat.No | ACP02062 | Target Name | POLQ |
|---|---|---|---|
| Target Synonyms | DNA polymerase eta | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 321-554aa |
| Mol Weight | 33.3 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O75417 |
|---|
Uniprot Id
O75417
Target Species
Human
Target Name
POLQ
Target Full Name
DNA polymerase theta
Target Function
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends. POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining. Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs.
Target Involvement
Breast cancer (BC)
Target Subcellular Location
Nucleus. Chromosome.
Target Protein Families
DNA polymerase type-A family
Target Tissue Specificity
Highly expressed in testis.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
(DNA polymerase eta)
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