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The expression region of this recombinant Human NEDD4L covers amino acids 2-975. The theoretical molecular weight of the NEDD4L protein is 138.5 kDa. Expression of this NEDD4L protein is conducted in e.coli. The NEDD4L coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant NEDD4L protein in following stages of expression and purification.The human E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) is a member of the NEDD4 family of HECT-type ubiquitin ligases. NEDD4L plays a key role in the regulation of protein degradation through the ubiquitin-proteasome system. It catalyzes the transfer of ubiquitin molecules onto substrate proteins, leading to their tagging for degradation. NEDD4L has been implicated in various cellular processes, including the control of cell growth, proliferation, and membrane trafficking. It interacts with a diverse array of substrates, including membrane proteins and signaling molecules, contributing to the regulation of multiple cellular pathways. NEDD4L's involvement in the ubiquitin-mediated protein degradation pathway makes it a crucial player in maintaining cellular homeostasis and responding to various physiological signals. Understanding NEDD4L's functions is essential for unraveling its roles in normal cellular processes and its potential implications in diseases.
The expression region of this recombinant Human NEDD4L covers amino acids 2-975. The theoretical molecular weight of the NEDD4L protein is 138.5 kDa. Expression of this NEDD4L protein is conducted in e.coli. The NEDD4L coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant NEDD4L protein in following stages of expression and purification.The human E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) is a member of the NEDD4 family of HECT-type ubiquitin ligases. NEDD4L plays a key role in the regulation of protein degradation through the ubiquitin-proteasome system. It catalyzes the transfer of ubiquitin molecules onto substrate proteins, leading to their tagging for degradation. NEDD4L has been implicated in various cellular processes, including the control of cell growth, proliferation, and membrane trafficking. It interacts with a diverse array of substrates, including membrane proteins and signaling molecules, contributing to the regulation of multiple cellular pathways. NEDD4L’s involvement in the ubiquitin-mediated protein degradation pathway makes it a crucial player in maintaining cellular homeostasis and responding to various physiological signals. Understanding NEDD4L’s functions is essential for unraveling its roles in normal cellular processes and its potential implications in diseases.
| Cat.No | ACP01319 | Target Name | NEDD4L |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-975aa | Mol Weight | 138.5 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q96PU5 |
|---|
Uniprot Id
Q96PU5
Target Species
Human
Target Name
NEDD4L
Target Full Name
E3 ubiquitin-protein ligase NEDD4-like
Target Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF.
Target Involvement
Periventricular nodular heterotopia 7 (PVNH7)
Target Subcellular Location
Cytoplasm. Golgi apparatus. Endosome, multivesicular body. Note=May be recruited to exosomes by NDFIP1.
Target Tissue Specificity
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.
Target Research Area
Cell Biology
Target Synonyms
E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed; developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5
Target Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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