Recombinant Human Elongin-B (ELOB)

The expression region of this recombinant Human ELOB covers amino acids 1-118. The expected molecular weight for the ELOB protein is calculated to be 40.1 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal GST tag into the ELOB encoding gene fragment was conducted, allowing for easier detection and purification of the ELOB protein in subsequent stages.The human Elongin B (ELOB) protein is a subunit of the Elongin complex, critical for RNA polymerase II transcription elongation. ELOB, along with Elongin C and Elongin A, forms a scaffold that interacts with transcription factors and regulates gene expression. In cancer research, ELOB is implicated in tumor development and progression, acting as a potential prognostic marker. Studying ELOB provides insights into transcriptional control mechanisms. Additionally, ELOB plays a role in viral replication, making it relevant in virology. Investigating ELOB's diverse functions enhances our understanding of transcriptional regulation, offering potential applications in cancer diagnostics, therapeutics, and antiviral strategies.

ACP03334

The expression region of this recombinant Human ELOB covers amino acids 1-118. The expected molecular weight for the ELOB protein is calculated to be 40.1 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal GST tag into the ELOB encoding gene fragment was conducted, allowing for easier detection and purification of the ELOB protein in subsequent stages.The human Elongin B (ELOB) protein is a subunit of the Elongin complex, critical for RNA polymerase II transcription elongation. ELOB, along with Elongin C and Elongin A, forms a scaffold that interacts with transcription factors and regulates gene expression. In cancer research, ELOB is implicated in tumor development and progression, acting as a potential prognostic marker. Studying ELOB provides insights into transcriptional control mechanisms. Additionally, ELOB plays a role in viral replication, making it relevant in virology. Investigating ELOB’s diverse functions enhances our understanding of transcriptional regulation, offering potential applications in cancer diagnostics, therapeutics, and antiviral strategies.

Number

Cat.No	ACP03334	Target Name	ELOB
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-118aa	Mol Weight	40.1kDa
Protein Length	Full length	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP03334

Target Name

ELOB

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-118aa

Mol Weight

40.1kDa

Protein Length

Full length

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q15370

Background Information

Uniprot Id

Q15370
Target Species

Human
Target Name

ELOB
Target Full Name

Elongin-B
Target Function

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.; Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. This includes the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation.
Target Subcellular Location

Nucleus.
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

EloB; ELOB_HUMAN; Elongin 18 kDa subunit; Elongin B; Elongin-B; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII; SIII p18; TCEB 2; Tceb2; Transcription elongation factor B (SIII) polypeptide 2; Transcription elongation factor B polypeptide 2
Target Background

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Recombinant Human Elongin-B (ELOB)

Specifications

Immunogen Information

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