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Constructing a plasmid that codes for the Human ESAM protein (30-248aa) is the initial step to yield the recombinant Human ESAM protein. The plasmid is then transferred into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. A N-terminal 6xHis-SUMO tag is fused to the protein. The affinity purification is used to purify the protein, and SDS-PAGE analysis is carried out to verify the presence and assess the purity of the protein. The protein possesses a purity exceeding 90%.
Constructing a plasmid that codes for the Human ESAM protein (30-248aa) is the initial step to yield the recombinant Human ESAM protein. The plasmid is then transferred into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. A N-terminal 6xHis-SUMO tag is fused to the protein. The affinity purification is used to purify the protein, and SDS-PAGE analysis is carried out to verify the presence and assess the purity of the protein. The protein possesses a purity exceeding 90%.
| Cat.No | ACP04128 | Target Name | ESAM |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 30-248aa | Mol Weight | 39.8kDa |
| Protein Length | Extracellular Domain | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q96AP7 |
|---|
Uniprot Id
Q96AP7
Target Species
Human
Target Name
ESAM
Target Full Name
Endothelial cell-selective adhesion molecule
Target Function
Can mediate aggregation most likely through a homophilic molecular interaction.
Target Subcellular Location
Cell junction, adherens junction. Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein.
Target Tissue Specificity
Highly expressed in endothelial cells.
Target Research Area
Cell Adhesion
Target Synonyms
2310008D05Rik; Endothelial cell adhesion molecule; Endothelial cell selective adhesion molecule; Endothelial cell-selective adhesion molecule; Esam; ESAM_HUMAN; HUEL (C4orf1) interacting protein ; LP4791 protein ; W117m
Target Background
Enables cell-cell adhesion mediator activity. Involved in several processes, including bicellular tight junction assembly; cell-cell adhesion; and regulation of actin cytoskeleton organization. Located in cell-cell junction and plasma membrane. Part of protein-containing complex.
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