Recombinant Human ER membrane protein complex subunit 10 (EMC10), Truncated

The creation of the recombinant Human EMC10 protein is achieved through genetic engineering techniques. A specific DNA sequence coding for the Human EMC10 protein (26-221aa) is integrated into an expression vector, which serves as a carrier for gene expression. This vector is then introduced into e.coli cells, which are cultured to facilitate the expression of the desired protein. The protein is fused with a N-terminal 6xHis-SUMO tag. The recombinant Human EMC10 protein is subsequently purified using affinity purification, resulting in a purity level exceeding 90%, as verified by SDS-PAGE analysis.

ACP01018

Number

Cat.No	ACP01018	Target Name	EMC10
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	26-221aa	Mol Weight	33.9 kDa
Protein Length	Partial	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01018

Target Name

EMC10

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

26-221aa

Mol Weight

33.9 kDa

Protein Length

Partial

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q5UCC4

Background Information

Uniprot Id

Q5UCC4
Target Species

Human
Target Name

EMC10
Target Full Name

ER membrane protein complex subunit 10
Target Function

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways
Target Subcellular Location

[Isoform 1]: Endoplasmic reticulum membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Target Protein Families

EMC10 family
Target Tissue Specificity

Present in serum (at protein level). Increased expression seen in the left ventrice after myocardial infarction (at protein level). Expressed in the pituitary gland; very low levels in other brain regions.
Target Research Area

Cell Biology
Target Synonyms

Chromosome 19 open reading frame 63; emc10; EMC10_HUMAN; ER membrane protein complex subunit 10; Hematopoietic signal peptide containing membrane domain containing 1; Hematopoietic signal peptide containing membrane domain containing protein 1; Hematopoietic signal peptide containing secreted 1; Hematopoietic signal peptide-containing membrane domain-containing protein 1; HSM1; HSS1; INM02; UPF0510 protein INM02

Recombinant Human ER membrane protein complex subunit 10 (EMC10), Truncated

Specifications

Immunogen Information

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