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Recombinant Human EIF3F protein expression is initiated by transfecting e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (2-357aa). The subsequent culturing of the cells induces the expression of the desired protein. The protein is equipped with a N-terminal 10xHis tag. The resulting recombinant Human EIF3F protein is collected and purified from the cell lysate through affinity purification, demonstrating a purity greater than 90%, as indicated by SDS-PAGE.
Recombinant Human EIF3F protein expression is initiated by transfecting e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (2-357aa). The subsequent culturing of the cells induces the expression of the desired protein. The protein is equipped with a N-terminal 10xHis tag. The resulting recombinant Human EIF3F protein is collected and purified from the cell lysate through affinity purification, demonstrating a purity greater than 90%, as indicated by SDS-PAGE.
| Cat.No | ACP01682 | Target Name | EIF3F |
|---|---|---|---|
| Target Synonyms | (eIF3f)(Deubiquitinating enzyme eIF3f)(Eukaryotic translation initiation factor 3 subunit 5)(eIF-3-epsilon)(eIF3 p47) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-357aa |
| Mol Weight | 43.5 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O00303 |
|---|
Uniprot Id
O00303
Target Species
Human
Target Name
EIF3F
Target Full Name
Eukaryotic translation initiation factor 3 subunit F
Target Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.; Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
Target Subcellular Location
Cytoplasm.
Target Protein Families
EIF-3 subunit F family
Target Research Area
Metabolism
Target Synonyms
Deubiquitinating enzyme eIF3f; eIF 3 epsilon; eIF-3-epsilon; eIF3 p47; eIF3 p47 subunit; eIF3-epsilon; eIF3-p47; eIF3f; EIF3F_HUMAN; EIF3S5; EIF3S5; formerly; Eukaryotic translation initiation factor 3 subunit 5; Eukaryotic translation initiation factor 3 subunit 5; formerly; Eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3; subunit 5 (epsilon; 47kD); eukaryotic translation initiation factor 3; subunit 5 epsilon; 47kDa; eukaryotic translation initiation factor 3; subunit F; translation initiation factor 3 47 kda subunit
Target Background
Enables deubiquitinase activity and identical protein binding activity. Contributes to translation initiation factor activity. Involved in IRES-dependent viral translational initiation; protein deubiquitination; and translational initiation. Located in membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in autosomal recessive non-syndromic intellectual disability.
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