Recombinant Human FAS-associated death domain protein (FADD)

The generation of the recombinant Human FADD protein involves manipulating gene expression in e.coli cells. A specific DNA sequence coding for the Human FADD protein (1-208aa) is inserted to an expression vector, which is then introduced into e.coli cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A C-terminal 6xHis tag is attached to the protein. The recombinant Human FADD protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.

ACP01078

Number

Cat.No	ACP01078	Target Name	FADD
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-208aa	Mol Weight	30.2 kDa
Protein Length	Full length	Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01078

Target Name

FADD

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-208aa

Mol Weight

30.2 kDa

Protein Length

Full length

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q13158

Background Information

Uniprot Id

Q13158
Target Species

Human
Target Name

FADD
Target Full Name

FAS-associated death domain protein
Target Function

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Target Involvement

Infections, recurrent, associated with encephalopathy, hepatic dysfunction and cardiovascular malformations (IEHDCM)
Target Tissue Specificity

Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Target Research Area

Others
Target Synonyms

FADD; FADD protein; FADD_HUMAN; Fas (TNFRSF6) associated via death domain; Fas associated via death domain; Fas associating death domain containing protein; Fas associating protein; Fas associating protein with death domain; Fas TNFRSF6 associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG 3; GIG3; Growth inhibiting gene 3 protein; Growth-inhibiting gene 3 protein; H sapiens mRNA for mediator of receptor induced toxicity; Mediator of receptor induced toxicity; MGC8528; MORT 1; MORT1; Protein FADD
Target Background

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Recombinant Human FAS-associated death domain protein (FADD)

Specifications

Immunogen Information

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