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The gene encoding the Human FGF10 protein (38-208aa) creates recombinant plasmid, which is then transformed into e.coli cells. e.coli cells that can endure a specific antibiotic are selected and cultured under conditions that encourage the expression of the gene of interest. The protein features a N-terminal GST tag fusion. Following expression, affinity purification is employed to isolate and purify the recombinant Human FGF10 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human FGF10 protein, indicating a purity level exceeding 90%.
The gene encoding the Human FGF10 protein (38-208aa) creates recombinant plasmid, which is then transformed into e.coli cells. e.coli cells that can endure a specific antibiotic are selected and cultured under conditions that encourage the expression of the gene of interest. The protein features a N-terminal GST tag fusion. Following expression, affinity purification is employed to isolate and purify the recombinant Human FGF10 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human FGF10 protein, indicating a purity level exceeding 90%.
| Cat.No | ACP04197 | Target Name | FGF10 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 38-208aa | Mol Weight | 46.3kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O15520 |
|---|
Uniprot Id
O15520
Target Species
Human
Target Name
FGF10
Target Full Name
Fibroblast growth factor 10
Target Function
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.
Target Involvement
Aplasia of lacrimal and salivary glands (ALSG); Lacrimo-auriculo-dento-digital syndrome (LADDS)
Target Subcellular Location
Secreted.
Target Protein Families
Heparin-binding growth factors family
Target Research Area
Cardiovascular
Target Synonyms
BB213776 ; fd11d03; FGF 10; FGF-10; FGF10; FGF10_HUMAN; Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF 2; Produced by fibroblasts of urinary bladder lamina propria; wu:fd11d03; zgc:109774
Target Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.
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