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The region for expressing recombinant Human FEN1 contains amino acids 1-380. This FEN1 protein is expected to have a theoretical molecular weight of 50.0 kDa. Expression of this FEN1 protein is conducted in e.coli. The FEN1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant FEN1 protein during the following stages.Flap endonuclease 1 (FEN1) is a multifunctional endonuclease involved in DNA replication and repair processes. In humans, FEN1 plays a crucial role in processing DNA intermediates during Okazaki fragment maturation, long-patch base excision repair, and nucleotide excision repair. As a structure-specific nuclease, FEN1 cleaves 5' flaps, flap structures that arise during DNA synthesis. This activity is vital for maintaining genomic stability and integrity. FEN1 also participates in DNA damage response pathways, contributing to the repair of various lesions. Dysregulation of FEN1 has been associated with genomic instability and is implicated in cancer development. Understanding FEN1's functions is essential for unraveling DNA repair mechanisms and potential therapeutic targets for cancer treatment.
The region for expressing recombinant Human FEN1 contains amino acids 1-380. This FEN1 protein is expected to have a theoretical molecular weight of 50.0 kDa. Expression of this FEN1 protein is conducted in e.coli. The FEN1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant FEN1 protein during the following stages.Flap endonuclease 1 (FEN1) is a multifunctional endonuclease involved in DNA replication and repair processes. In humans, FEN1 plays a crucial role in processing DNA intermediates during Okazaki fragment maturation, long-patch base excision repair, and nucleotide excision repair. As a structure-specific nuclease, FEN1 cleaves 5′ flaps, flap structures that arise during DNA synthesis. This activity is vital for maintaining genomic stability and integrity. FEN1 also participates in DNA damage response pathways, contributing to the repair of various lesions. Dysregulation of FEN1 has been associated with genomic instability and is implicated in cancer development. Understanding FEN1’s functions is essential for unraveling DNA repair mechanisms and potential therapeutic targets for cancer treatment.
| Cat.No | ACP04939 | Target Name | FEN1 |
|---|---|---|---|
| Target Synonyms | DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-380aa |
| Mol Weight | 50.0 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P39748 |
|---|
Uniprot Id
P39748
Target Species
Human
Target Name
FEN1
Target Full Name
Flap endonuclease 1
Target Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Target Subcellular Location
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion.
Target Protein Families
XPG/RAD2 endonuclease family, FEN1 subfamily
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2
Target Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
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