Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1), Truncated

Uniprot Id

Q9P2W7

Target Species

Human

Target Name

B3GAT1

Target Full Name

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Target Function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Target Subcellular Location

[Isoform 1]: Golgi apparatus membrane; Single-pass type II membrane protein. Secreted.; [Isoform 2]: Golgi apparatus membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Secreted.

Target Protein Families

Glycosyltransferase 43 family

Target Tissue Specificity

Mainly expressed in the brain.

Target Synonyms

3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; B3gat1; Beta 1 3 glucuronyltransferase 1; Beta-1; Beta-1,3-glucuronyltransferase 1; CD 57; CD57; CD57 antigen; Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU 7; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1

Target Background

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.