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In the production of the recombinant Human GOLPH3 protein in e.coli cells, a sequential pipeline involves constructing the expression vector coding for the Human GOLPH3 protein (1-298aa), transforming the recombinant vector into e.coli cells, selecting the positive cells and cultivating them, lysing cells, and characterizing the protein. The resulting recombinant Human GOLPH3 protein is purified from the cell lysate using affinity purification. Its purity exceeds 85%, as determined by SDS-PAGE.
In the production of the recombinant Human GOLPH3 protein in e.coli cells, a sequential pipeline involves constructing the expression vector coding for the Human GOLPH3 protein (1-298aa), transforming the recombinant vector into e.coli cells, selecting the positive cells and cultivating them, lysing cells, and characterizing the protein. The resulting recombinant Human GOLPH3 protein is purified from the cell lysate using affinity purification. Its purity exceeds 85%, as determined by SDS-PAGE.
| Cat.No | ACP02078 | Target Name | GOLPH3 |
|---|---|---|---|
| Target Synonyms | Coat protein GPP34 (Mitochondrial DNA absence factor) (MIDAS) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-298aa |
| Mol Weight | 39.8 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9H4A6 |
|---|
Uniprot Id
Q9H4A6
Target Species
Human
Target Name
GOLPH3
Target Full Name
Golgi phosphoprotein 3
Target Function
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
Target Subcellular Location
Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome.
Target Protein Families
GOLPH3/VPS74 family
Target Tissue Specificity
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Target Research Area
Signal Transduction
Target Synonyms
Coat protein; Coat protein GPP34; FLJ90675; Golgi associated protein; Golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3 (coat protein); Golgi phosphoprotein 3; Golgi protein; GOLP3_HUMAN; Golph3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor
Target Background
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
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