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The recombinant Human GDF2 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human GDF2 protein (300-429aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the GDF2 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human GDF2 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human GDF2 protein reaches over 90%.
The recombinant Human GDF2 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human GDF2 protein (300-429aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the GDF2 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human GDF2 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human GDF2 protein reaches over 90%.
| Cat.No | ACP04529 | Target Name | GDF2 |
|---|---|---|---|
| Target Synonyms | GDF2; BMP9Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9 | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Expression Range | 300-429aa |
| Mol Weight | 16.3kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9UK05 |
|---|
Uniprot Id
Q9UK05
Target Species
Human
Target Name
GDF2
Target Full Name
Growth/differentiation factor 2
Target Function
Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
Target Involvement
Telangiectasia, hereditary hemorrhagic, 5 (HHT5)
Target Subcellular Location
Secreted.
Target Protein Families
TGF-beta family
Target Tissue Specificity
Detected in blood plasma (at protein level).
Target Research Area
Cell Biology
Target Synonyms
GDF2; BMP9Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9
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