Recombinant Human Growth/differentiation factor 9 (GDF9)

The gene responsible for the Human GDF9 protein (320-454aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal GST tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human GDF9 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human GDF9 protein, revealing a purity greater than 90%.

ACP04253

Number

Cat.No	ACP04253	Target Name	GDF9
Target Synonyms	GDF9Growth/differentiation factor 9; GDF-9	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	320-454aa
Mol Weight	42.5kDa	Protein Length	Full Length of Mature Protein
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04253

Target Name

GDF9

Target Synonyms

GDF9Growth/differentiation factor 9; GDF-9

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

320-454aa

Mol Weight

42.5kDa

Protein Length

Full Length of Mature Protein

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O60383

Background Information

Uniprot Id

O60383
Target Species

Human
Target Name

GDF9
Target Full Name

Growth/differentiation factor 9
Target Function

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Target Involvement

Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins.
Target Subcellular Location

Secreted.
Target Protein Families

TGF-beta family
Target Tissue Specificity

Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
Target Research Area

Cardiovascular
Target Synonyms

GDF9Growth/differentiation factor 9; GDF-9
Target Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins.

Recombinant Human Growth/differentiation factor 9 (GDF9)

Specifications

Immunogen Information

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