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The expression of recombinant Human HELQ protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (350-726aa). After transfection, the cells are cultured to produce the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human HELQ protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 90%, as determined by SDS-PAGE.
The expression of recombinant Human HELQ protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (350-726aa). After transfection, the cells are cultured to produce the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human HELQ protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 90%, as determined by SDS-PAGE.
| Cat.No | ACP01236 | Target Name | HELQ |
|---|---|---|---|
| Target Synonyms | Mus308-like helicase;POLQ-like helicase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 350-726aa |
| Mol Weight | 49.8 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8TDG4 |
|---|
Uniprot Id
Q8TDG4
Target Species
Human
Target Name
HELQ
Target Full Name
Helicase POLQ-like
Target Function
Single-stranded DNA-dependent ATPase and 5' to 3' DNA helicase. Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with POLN polymerase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links.
Target Protein Families
Helicase family, SKI2 subfamily
Target Research Area
Others
Target Synonyms
HEL308 protein; Helicase POLQ-like; Helicase, POLQ like; HELQ; HELQ_HUMAN; Mus308 like helicase; Mus308-like helicase; POLQ like helicase; POLQ-like helicase
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