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The recombinant Human PDE9A protein production is accomplished by manipulating the expression of the desired gene in e.coli cells. The sequence (1-593aa) of foreign DNA is incorporated into an expression vector and subsequently introduced into e.coli cells. Positive cells are selected and cultured to induce the expression of the desired protein. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. The resulting recombinant Human PDE9A protein undergoes affinity purification, with a purity surpassing 85%, as measured by SDS-PAGE.
The recombinant Human PDE9A protein production is accomplished by manipulating the expression of the desired gene in e.coli cells. The sequence (1-593aa) of foreign DNA is incorporated into an expression vector and subsequently introduced into e.coli cells. Positive cells are selected and cultured to induce the expression of the desired protein. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. The resulting recombinant Human PDE9A protein undergoes affinity purification, with a purity surpassing 85%, as measured by SDS-PAGE.
| Cat.No | ACP01041 | Target Name | PDE9A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-593aa | Mol Weight | 76.0 kDa |
| Protein Length | Full length | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O76083 |
|---|
Uniprot Id
O76083
Target Species
Human
Target Name
PDE9A
Target Full Name
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Target Function
Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory.
Target Subcellular Location
[Isoform PDE9A1]: Cell projection, ruffle membrane. Cytoplasm, perinuclear region. Golgi apparatus. Endoplasmic reticulum. Cell membrane, sarcolemma.; [Isoform PDE9A2]: Cell projection, ruffle membrane. Cytoplasm, perinuclear region.; [Isoform PDE9A3]: Cytoplasm. Endoplasmic reticulum.; [Isoform PDE9A17]: Cytoplasm. Endoplasmic reticulum.
Target Protein Families
Cyclic nucleotide phosphodiesterase family, PDE9 subfamily
Target Tissue Specificity
Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isofo
Target Research Area
Others
Target Synonyms
5''-cyclic phosphodiesterase 9A; cGMP specific 3' 5' cyclic phosphodiesterase type 9; FLJ90181; High affinity cGMP-specific 3''; High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A; HSPDE9A2; OTTHUMP00000109399; PDE 9A; Pde9a; PDE9A_HUMAN; Phosphodiesterase 9A ; phosphodiesterase PDE9A21
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