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The generation of the recombinant Human IL12RB1 protein involves manipulating gene expression in baculovirus cells. A specific DNA sequence coding for the Human IL12RB1 protein (24-545aa) is inserted to an expression vector, which is then introduced into baculovirus cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A C-terminal 6xHis tag is attached to the protein. The recombinant Human IL12RB1 protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.
The generation of the recombinant Human IL12RB1 protein involves manipulating gene expression in baculovirus cells. A specific DNA sequence coding for the Human IL12RB1 protein (24-545aa) is inserted to an expression vector, which is then introduced into baculovirus cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A C-terminal 6xHis tag is attached to the protein. The recombinant Human IL12RB1 protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.
| Cat.No | ACP01032 | Target Name | IL12RB1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Baculovirus |
| Expression Range | 24-545aa | Mol Weight | 60.4 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P42701 |
|---|
Uniprot Id
P42701
Target Species
Human
Target Name
IL12RB1
Target Full Name
Interleukin-12 receptor subunit beta-1
Target Function
Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Target Involvement
Immunodeficiency 30 (IMD30)
Target Subcellular Location
Membrane; Single-pass type I membrane protein.
Target Protein Families
Type I cytokine receptor family, Type 2 subfamily
Target Research Area
Immunology
Target Synonyms
IL12RB1; IL12R; IL12RB; Interleukin-12 receptor subunit beta-1; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1; IL-12 receptor beta component; CD antigen CD212
Target Background
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.
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