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The recombinant human IL18 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transfected into the yeast cells that support the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag gene to the gene fragment coding for the 1-193aa of the human IL18 protein. After purification, the product is the recombinant human IL18 protein. This recombinant IL18 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This IL18 protein ran to the molecular weight of about 25 kDa under SDS-PAGE condition. It may find use in immunology research.Interleukin-18 (IL-18) belongs to the IL-1 family of cytokines. The inactive IL-18 precursor is synthesized as its active form by caspase-1. In humans, increased disease severity can be associated with an imbalance of IL-18. IL-18 has been found to be associated with the injury of different organs. Furthermore, the overly imbalance of cytokines could cause potentially fatal conditions such as a cytokine storm. IL-18 forms a signaling complex by binding to the IL-18 alpha chain. It has been reported that IL-18 is associated with hyperphagia and a variety of metabolic syndrome. Importantly, increasing numbers of animal and clinical studies indicate a role for IL-18 in heart disease.
The recombinant human IL18 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transfected into the yeast cells that support the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag gene to the gene fragment coding for the 1-193aa of the human IL18 protein. After purification, the product is the recombinant human IL18 protein. This recombinant IL18 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This IL18 protein ran to the molecular weight of about 25 kDa under SDS-PAGE condition. It may find use in immunology research.Interleukin-18 (IL-18) belongs to the IL-1 family of cytokines. The inactive IL-18 precursor is synthesized as its active form by caspase-1. In humans, increased disease severity can be associated with an imbalance of IL-18. IL-18 has been found to be associated with the injury of different organs. Furthermore, the overly imbalance of cytokines could cause potentially fatal conditions such as a cytokine storm. IL-18 forms a signaling complex by binding to the IL-18 alpha chain. It has been reported that IL-18 is associated with hyperphagia and a variety of metabolic syndrome. Importantly, increasing numbers of animal and clinical studies indicate a role for IL-18 in heart disease.
| Cat.No | ACP02222 | Target Name | IL18 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 1-193aa | Mol Weight | 24.8kDa |
| Protein Length | Full?Length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q14116 |
|---|
Uniprot Id
Q14116
Target Species
Human
Target Name
IL18
Target Full Name
Interleukin-18
Target Function
A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable).
Target Subcellular Location
Cytoplasm. Secreted.
Target Protein Families
IL-1 family
Target Research Area
Immunology
Target Synonyms
Iboctadekin; IFN gamma inducing factor; IFN-gamma-inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL-1 gamma; IL-18; IL1 gamma; IL18; IL18 protein; IL18_HUMAN; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interferon gamma-inducing factor; Interleukin 1 gamma; Interleukin 18 (interferon-gamma-inducing factor); Interleukin 18; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Target Background
The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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