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Inserting the gene encoding the Human IL3 protein (20-152aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A C-terminal 6xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human IL3 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human IL3 protein, revealing a purity level exceeding 85%.
Inserting the gene encoding the Human IL3 protein (20-152aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A C-terminal 6xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human IL3 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human IL3 protein, revealing a purity level exceeding 85%.
| Cat.No | ACP02480 | Target Name | IL3 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 20-152aa | Mol Weight | 17.1 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P08700 |
|---|
Uniprot Id
P08700
Target Species
Human
Target Name
IL3
Target Full Name
Interleukin-3
Target Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Target Subcellular Location
Secreted.
Target Protein Families
IL-3 family
Target Tissue Specificity
Activated T-cells, mast cells, natural killer cells.
Target Research Area
Immunology
Target Synonyms
Colony stimulating factor multiple; Hematopoietic growth factor; IL 3; IL-3; IL3; IL3_HUMAN; Interleukin 3 (colony stimulating factor; multiple); Interleukin 3; Interleukin-3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; Multipotential colony-stimulating factor; OTTHUMP00000065963; P cell stimulating factor ; P-cell-stimulating factor
Target Background
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
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