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Inserting the gene encoding the Human KRT38 protein (2-456aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human KRT38 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human KRT38 protein, revealing a purity level exceeding 90%.
Inserting the gene encoding the Human KRT38 protein (2-456aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human KRT38 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human KRT38 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP04373 | Target Name | KRT38 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-456aa | Mol Weight | 66.3kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O76015 |
|---|
Uniprot Id
O76015
Target Species
Human
Target Name
KRT38
Target Full Name
Keratin, type I cuticular Ha8
Target Research Area
Signal Transduction
Target Synonyms
HA8; Hair keratin; Hair keratin; type I Ha8; HHa8; HKA8; K38; Keratin 38; Keratin; Keratin; hair; acidic; 8; Keratin; type I cuticular Ha8; Keratin-38; KRT38; KRT38_HUMAN; KRTHA8; type I cuticular Ha8; type I Ha8
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