Recombinant Human Merlin (NF2)

The recombinant Human NF2 was expressed with the amino acid range of 1-595. The theoretical molecular weight of the NF2 protein is 73.7 kDa. This NF2 recombinant protein is manufactured in e.coli. The NF2 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant NF2 protein in following stages of expression and purification.Merlin, also known as neurofibromin 2 (NF2), is a tumor suppressor protein that plays a critical role in regulating cell proliferation and growth. NF2 belongs to the Ezrin/Radixin/Moesin (ERM) protein family and is primarily found at the cell membrane, where it interacts with various membrane-associated proteins. NF2 is involved in cell adhesion, motility, and signaling pathways. Its main function is to suppress the development of tumors by inhibiting cell division and promoting contact-dependent inhibition of proliferation. Mutations in the NF2 gene can lead to neurofibromatosis type 2, a genetic disorder characterized by the development of benign tumors in the nervous system. Research on NF2 spans multiple areas, including cancer biology, cell signaling, and the development of therapeutic strategies for NF2-related conditions.

ACP02312

Number

Cat.No	ACP02312	Target Name	NF2
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-595aa	Mol Weight	73.7 kDa
Protein Length	Full length	Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP02312

Target Name

NF2

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-595aa

Mol Weight

73.7 kDa

Protein Length

Full length

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P35240

Background Information

Uniprot Id

P35240
Target Species

Human
Target Name

NF2
Target Full Name

Merlin
Target Function

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Target Involvement

Neurofibromatosis 2 (NF2); Schwannomatosis 1 (SWNTS1); Mesothelioma, malignant (MESOM)
Target Subcellular Location

[Isoform 1]: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.; [Isoform 7]: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia.; [Isoform 9]: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.; [Isoform 10]: Nucleus. Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region. Cytoplasmic granule. Cytoplasm, cytoskeleton. Note=In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia.
Target Tissue Specificity

Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adul
Target Research Area

Cancer
Target Synonyms

ACN; BANF; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radixin like protein; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis 2; Neurofibromatosis type 2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin-2; Neurofibromin2; NF 2; Nf2; SCH; Schwannomerlin; Schwannomin
Target Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.

Recombinant Human Merlin (NF2)

Specifications

Immunogen Information

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