Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The process of expressing the recombinant Human PLD6 protein includes transfecting e.coli cells with a DNA expression vector carrying the gene for the desired protein (1-252aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is equipped with a N-terminal 6xHis tag. The recombinant Human PLD6 protein is then collected and purified from the cell lysate through affinity purification, exhibiting a purity exceeding 85%, as verified by SDS-PAGE.
The process of expressing the recombinant Human PLD6 protein includes transfecting e.coli cells with a DNA expression vector carrying the gene for the desired protein (1-252aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is equipped with a N-terminal 6xHis tag. The recombinant Human PLD6 protein is then collected and purified from the cell lysate through affinity purification, exhibiting a purity exceeding 85%, as verified by SDS-PAGE.
| Cat.No | ACP01718 | Target Name | PLD6 |
|---|---|---|---|
| Target Synonyms | (Choline phosphatase 6)(Mitochondrial phospholipase)(MitoPLD)(Phosphatidylcholine-hydrolyzing phospholipase D6)(Phospholipase D6)(PLD 6)(Protein zucchini homolog) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-252aa |
| Mol Weight | 31.0 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8N2A8 |
|---|
Uniprot Id
Q8N2A8
Target Species
Human
Target Name
PLD6
Target Full Name
Mitochondrial cardiolipin hydrolase
Target Function
Presents phospholipase and nuclease activities, depending on the different physiological conditions. Interaction with Mitoguardin (MIGA1 or MIGA2) affects the dimer conformation, facilitating the lipase activity over the nuclease activity. Plays a key role in mitochondrial fusion and fission via its phospholipase activity. In its phospholipase role, it uses the mitochondrial lipid cardiolipin as substrate to generate phosphatidate (PA or 1,2-diacyl-sn-glycero-3-phosphate), a second messenger signaling lipid. Production of PA facilitates Mitofusin-mediated fusion, whereas the cleavage of PA by the Lipin family of phosphatases produces diacylgycerol (DAG) which promotes mitochondrial fission. Both Lipin and DAG regulate mitochondrial dynamics and membrane fusion/fission, important processes for adapting mitochondrial metabolism to changes in cell physiology. Mitochondrial fusion enables cells to cope with the increased nucleotide demand during DNA synthesis. Mitochondrial function and dynamics are closely associated with biological processes such as cell growth, proliferation, and differentiation. Mediator of MYC activity, promotes mitochondrial fusion and activates AMPK which in turn inhibits YAP/TAZ, thereby inducing cell growth and proliferation. The endonuclease activity plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. Implicated in spermatogenesis and sperm fertility in testicular germ cells, its single strand-specific nuclease activity is critical for the biogenesis/maturation of PIWI-interacting RNA (piRNA). MOV10L1 selectively binds to piRNA precursors and funnels them to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins. Cleaves either DNA or RNA substrates with similar affinity, producing a 5' phosphate end, in this way it participates in the processing of primary piRNA transcripts. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. PA may act as signaling molecule in the recognition/transport of the precursor RNAs of primary piRNAs. Interacts with tesmin in testes, suggesting a role in spermatogenesis via association with its interacting partner.
Target Subcellular Location
Mitochondrion outer membrane; Single-pass membrane protein. Golgi apparatus.
Target Protein Families
Phospholipase D family, MitoPLD/Zucchini subfamily
Target Tissue Specificity
Predominantly expressed in testis and ovary, but not limited to gonads (at protein level). It is also found in brain, heart, pituitary gland, prostate, pancreas, thyroid, bone marrow, lung and muscle.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
Choline phosphatase 6; Mitochondrial cardiolipin hydrolase; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine hydrolyzing phospholipase D6; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D family member 6; Phospholipase D6; PLD 6; pld6; PLD6_HUMAN; Protein zucchini homolog; ZUC; Zucchini, Drosophila, homolog of
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
