Recombinant Human Mitotic checkpoint serine/threonine-protein kinase BUB1 (BUB1), Truncated

Uniprot Id

O43683

Target Species

Human

Target Name

BUB1

Target Full Name

Mitotic checkpoint serine/threonine-protein kinase BUB1

Target Function

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Target Subcellular Location

Nucleus. Chromosome, centromere, kinetochore.

Target Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily

Target Tissue Specificity

High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.

Target Synonyms

Bub1; BUB1 budding uninhibited by benzimidazoles 1 homolog ; BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast); BUB1 mitotic checkpoint serine/threonine kinase; BUB1; S. cerevisiae; homolog of; BUB1_HUMAN; BUB1A; BUB1L; Budding uninhibited by benzimidazoles 1 (yeast homolog); Budding uninhibited by benzimidazoles 1 homolog ; Budding uninhibited by benzimidazoles 1; S. cerevisiae; homolog of; hBUB1; Homolog of mitotic checkpoint gene BUB1; Mitotic checkpoint gene BUB1; Mitotic checkpoint serine/threonine protein kinase BUB1 ; Mitotic checkpoint serine/threonine-protein kinase BUB1; Mitotic spindle checkpoint kinase ; Putative serine/threonine protein kinase

Target Background

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.