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CUSABIO team inserts the gene coding for the Human NPHS1 protein (23-257aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human NPHS1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human NPHS1 protein, demonstrating a purity greater than 90%.
CUSABIO team inserts the gene coding for the Human NPHS1 protein (23-257aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human NPHS1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human NPHS1 protein, demonstrating a purity greater than 90%.
| Cat.No | ACP02513 | Target Name | NPHS1 |
|---|---|---|---|
| Target Synonyms | CNF ; Nephrin; Nephrosis 1 congenital Finnish type ; Nephrosis 1, congenital, Finnish type (nephrin); NPHN ; NPHN_HUMAN; NPHS 1; Nphs1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 23-257aa |
| Mol Weight | 29.1kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O60500 |
|---|
Uniprot Id
O60500
Target Species
Human
Target Name
NPHS1
Target Full Name
Nephrin
Target Function
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Target Involvement
Nephrotic syndrome 1 (NPHS1)
Target Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Target Protein Families
Immunoglobulin superfamily
Target Tissue Specificity
Specifically expressed in podocytes of kidney glomeruli.
Target Research Area
Cell Adhesion, Signal Transduction
Target Synonyms
CNF ; Nephrin; Nephrosis 1 congenital Finnish type ; Nephrosis 1, congenital, Finnish type (nephrin); NPHN ; NPHN_HUMAN; NPHS 1; Nphs1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor
Target Background
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
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