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Recombinant Human NF1 protein expression is initiated by transfecting e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (1566-1837aa). The subsequent culturing of the cells induces the expression of the desired protein. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. The resulting recombinant Human NF1 protein is collected and purified from the cell lysate through affinity purification, demonstrating a purity greater than 85%, as indicated by SDS-PAGE.
Recombinant Human NF1 protein expression is initiated by transfecting e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (1566-1837aa). The subsequent culturing of the cells induces the expression of the desired protein. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. The resulting recombinant Human NF1 protein is collected and purified from the cell lysate through affinity purification, demonstrating a purity greater than 85%, as indicated by SDS-PAGE.
| Cat.No | ACP01678 | Target Name | NF1 |
|---|---|---|---|
| Target Synonyms | (Neurofibromatosis-related protein NF-1) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1566-1837aa |
| Mol Weight | 38.7 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P21359 |
|---|
Uniprot Id
P21359
Target Species
Human
Target Name
NF1
Target Full Name
Neurofibromin
Target Function
Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
Target Involvement
Neurofibromatosis 1 (NF1); Leukemia, juvenile myelomonocytic (JMML); Watson syndrome (WTSN); Familial spinal neurofibromatosis (FSNF); Neurofibromatosis-Noonan syndrome (NFNS); Colorectal cancer (CRC)
Target Subcellular Location
Nucleus. Nucleus, nucleolus.
Target Tissue Specificity
Detected in brain, peripheral nerve, lung, colon and muscle.
Target Research Area
Cancer
Target Synonyms
DKFZp686J1293; FLJ21220; Neurofibromatosis Noonan syndrome; Neurofibromatosis related protein NF 1; Neurofibromatosis related protein NF1; neurofibromatosis type I; Neurofibromatosis-related protein NF-1; Neurofibromin 1; Neurofibromin truncated; Neurofibromin1; NF 1; NF; NF1; NF1_HUMAN; NFNS; Type 1 Neurofibromatosis; von Recklinghausen disease neurofibromin; von Recklinghausen disease related protein VRNF; VRNF; WATS; Watson disease related protein WSS; Watson syndrome; WSS
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