Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The recombinant human neurofilament light polypeptide (NEFL) is prepared in the E.coli. Its expression region lies in the fragment encoding amino acid residues 2-543 of the human NEFL protein. This recombinant human NEFL protein is labeled with a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. It harbors the full length of the mature protein in length. The purity of this NEFL protein is greater than 90% determined by SDS-PAGE. On the gel, this protein migrated to the band with a molecular weight of about 90 kDa. And its component has been identified by the LC-MS/MS Analysis. The NEFL protein is the light chain of neurofilaments that play an important role in axonal growth and the determination of axonal caliber. As axons are injured and die in neurodegenerative processes, NEFL leaks into the interstitial space, then into CSF and plasma. NEFL levels thus increase as neurodegenerative diseases progress and can reflect disease activity. Numerous studies have shown that NEFL is a potential biomarker for determining the stage of disease, tracking progression, and aiding in the identification of disease-modifying treatments in neurological disorders.
The recombinant human neurofilament light polypeptide (NEFL) is prepared in the E.coli. Its expression region lies in the fragment encoding amino acid residues 2-543 of the human NEFL protein. This recombinant human NEFL protein is labeled with a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. It harbors the full length of the mature protein in length. The purity of this NEFL protein is greater than 90% determined by SDS-PAGE. On the gel, this protein migrated to the band with a molecular weight of about 90 kDa. And its component has been identified by the LC-MS/MS Analysis.
The NEFL protein is the light chain of neurofilaments that play an important role in axonal growth and the determination of axonal caliber. As axons are injured and die in neurodegenerative processes, NEFL leaks into the interstitial space, then into CSF and plasma. NEFL levels thus increase as neurodegenerative diseases progress and can reflect disease activity. Numerous studies have shown that NEFL is a potential biomarker for determining the stage of disease, tracking progression, and aiding in the identification of disease-modifying treatments in neurological disorders.
| Cat.No | ACP02424 | Target Name | NEFL |
|---|---|---|---|
| Target Synonyms | NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-543aa |
| Mol Weight | 81.4kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P07196 |
|---|
Uniprot Id
P07196
Target Species
Human
Target Name
NEFL
Target Full Name
Neurofilament light polypeptide
Target Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Target Involvement
Charcot-Marie-Tooth disease 1F (CMT1F); Charcot-Marie-Tooth disease 2E (CMT2E)
Target Subcellular Location
Cell projection, axon. Cytoplasm, cytoskeleton.
Target Protein Families
Intermediate filament family
Target Research Area
Others
Target Synonyms
NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
Target Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
