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The generation of the recombinant Human NDE1 protein involves manipulating gene expression in e.coli cells. A specific DNA sequence coding for the Human NDE1 protein (1-335aa) is inserted to an expression vector, which is then introduced into e.coli cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. The recombinant Human NDE1 protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.
The generation of the recombinant Human NDE1 protein involves manipulating gene expression in e.coli cells. A specific DNA sequence coding for the Human NDE1 protein (1-335aa) is inserted to an expression vector, which is then introduced into e.coli cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. The recombinant Human NDE1 protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.
| Cat.No | ACP01077 | Target Name | NDE1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-335aa | Mol Weight | 45.2 kDa |
| Protein Length | Full length | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9NXR1 |
|---|
Uniprot Id
Q9NXR1
Target Species
Human
Target Name
NDE1
Target Full Name
Nuclear distribution protein nudE homolog 1
Target Function
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Target Involvement
Lissencephaly 4 (LIS4); Microhydranencephaly (MHAC)
Target Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Note=Localizes to the interphase and S phase centrosome. During mitosis, partially associated with the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis. manner. Also localizes to the cleavage furrow during cytokinesis.
Target Protein Families
NudE family
Target Tissue Specificity
Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
Target Research Area
Cell Biology
Target Synonyms
FLJ20101; HOM TES 87; LIS1 interacting protein NUDE1 rat homolog; LIS1 interacting protein NUDE1; LIS4; NDE 1; NDE1; NDE1_HUMAN; Nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NUDE 1; NudE; NudE nuclear distribution gene E homolog 1 (A. nidulans) ; NudE nuclear distribution gene E homolog 1; NUDE1
Target Background
This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants.
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