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CUSABIO team inserts the gene coding for the Human ODC1 protein (1-461aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human ODC1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human ODC1 protein, demonstrating a purity greater than 90%.
CUSABIO team inserts the gene coding for the Human ODC1 protein (1-461aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human ODC1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human ODC1 protein, demonstrating a purity greater than 90%.
| Cat.No | ACP02345 | Target Name | ODC1 |
|---|---|---|---|
| Target Synonyms | DCOR_HUMAN; Dodc1; Odc 1; ODC; Odc1; ODC2; Ornithine decarboxylase 1; Ornithine decarboxylase 2; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-461aa |
| Mol Weight | 71.1 kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P11926 |
|---|
Uniprot Id
P11926
Target Species
Human
Target Name
ODC1
Target Full Name
Ornithine decarboxylase
Target Function
Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.
Target Protein Families
Orn/Lys/Arg decarboxylase class-II family
Target Research Area
Signal Transduction
Target Synonyms
DCOR_HUMAN; Dodc1; Odc 1; ODC; Odc1; ODC2; Ornithine decarboxylase 1; Ornithine decarboxylase 2; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC
Target Background
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
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