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To achieve the expression of the recombinant Human NPFF protein in e.coli cells, a DNA fragment encoding the Human NPFF protein (66-76aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the NPFF protein. The protein features a N-terminal GST tag fusion. The recombinant Human NPFF protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human NPFF protein is greater than 90%, measured by SDS-PAGE.
To achieve the expression of the recombinant Human NPFF protein in e.coli cells, a DNA fragment encoding the Human NPFF protein (66-76aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the NPFF protein. The protein features a N-terminal GST tag fusion. The recombinant Human NPFF protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human NPFF protein is greater than 90%, measured by SDS-PAGE.
| Cat.No | ACP01968 | Target Name | NPFF |
|---|---|---|---|
| Target Synonyms | FMRFamide-related peptides | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 66-76aa |
| Mol Weight | 32.8 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O15130 |
|---|
Uniprot Id
O15130
Target Species
Human
Target Name
NPFF
Target Full Name
Pro-FMRFamide-related neuropeptide FF
Target Function
Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.
Target Subcellular Location
Secreted.
Target Protein Families
FARP (FMRFamide related peptide) family
Target Research Area
Neuroscience
Target Synonyms
NPFF; Pro-FMRFamide-related neuropeptide FF; FMRFamide-related peptides) [Cleaved into: Neuropeptide SF; NPSF); Neuropeptide FF; NPFF); Neuropeptide AF; NPAF)]
Target Background
This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
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