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CUSABIO team inserts the gene coding for the Human PFN1 protein (2-140aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal GST tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human PFN1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human PFN1 protein, demonstrating a purity greater than 90%.
CUSABIO team inserts the gene coding for the Human PFN1 protein (2-140aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal GST tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human PFN1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human PFN1 protein, demonstrating a purity greater than 90%.
| Cat.No | ACP02295 | Target Name | PFN1 |
|---|---|---|---|
| Target Synonyms | Actin binding protein ; ALS18; Epididymis tissue protein Li 184a; OTTHUMP00000125244; PFN 1; Pfn; PFN1; PROF1_HUMAN; Profilin I; Profilin-1; Profilin1; ProfilinI | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-140aa |
| Mol Weight | 41.9kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P07737 |
|---|
Uniprot Id
P07737
Target Species
Human
Target Name
PFN1
Target Full Name
Profilin-1
Target Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Target Involvement
Amyotrophic lateral sclerosis 18 (ALS18)
Target Subcellular Location
Cytoplasm, cytoskeleton.
Target Protein Families
Profilin family
Target Tissue Specificity
Expressed in epididymis (at protein level).
Target Research Area
Neuroscience
Target Synonyms
Actin binding protein ; ALS18; Epididymis tissue protein Li 184a; OTTHUMP00000125244; PFN 1; Pfn; PFN1; PROF1_HUMAN; Profilin I; Profilin-1; Profilin1; ProfilinI
Target Background
This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.
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