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The gene fragment corresponding to the 1-442aa of the human CRBN protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with C-terminal 9xHis tag. The Baculovirus was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as C-terminal 9xHis-tagged fusion. This recombinant human CRBN protein's purity is greater than 85% assayed by SDS-PAGE. The CRBN protein ran to a band of about 56 kDa molecular weight on the gel, indicating a glycosylated form of the protein.Cereblon (CRBN) is a common direct target of thalidomide and related compounds and works as a Cullin Ring 4 E3 ubiquitin ligase (CRL4) with DDB1, CUL4, and ROC1. CRBN the molecular target of lenalidomide and pomalidomide, is a substrate receptor of the cullin ring E 3 ubiquitin ligase complex, CRL 4 CRBN. In early reports, Researchers identifed that a novel cereblon modulator recruits GSPT1 to the CRL4-CRBN ubiquitin ligase. The substrate specificity of CRL4CRBN is modulated by thalidomide-related compounds. Ablation ofCRBNinduces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway. The CRBN, CUL4A and DDB1 expression predicts the response to immunomodulatory drugs and survival of multiple myeloma patients. Cereblon modulator CC-885 inducesCRBN-dependent ubiquitination and degradation of CDK4 in multiple myeloma.
The gene fragment corresponding to the 1-442aa of the human CRBN protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with C-terminal 9xHis tag. The Baculovirus was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as C-terminal 9xHis-tagged fusion. This recombinant human CRBN protein’s purity is greater than 85% assayed by SDS-PAGE. The CRBN protein ran to a band of about 56 kDa molecular weight on the gel, indicating a glycosylated form of the protein.Cereblon (CRBN) is a common direct target of thalidomide and related compounds and works as a Cullin Ring 4 E3 ubiquitin ligase (CRL4) with DDB1, CUL4, and ROC1. CRBN the molecular target of lenalidomide and pomalidomide, is a substrate receptor of the cullin ring E 3 ubiquitin ligase complex, CRL 4 CRBN. In early reports, Researchers identifed that a novel cereblon modulator recruits GSPT1 to the CRL4-CRBN ubiquitin ligase. The substrate specificity of CRL4CRBN is modulated by thalidomide-related compounds. Ablation ofCRBNinduces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway. The CRBN, CUL4A and DDB1 expression predicts the response to immunomodulatory drugs and survival of multiple myeloma patients. Cereblon modulator CC-885 inducesCRBN-dependent ubiquitination and degradation of CDK4 in multiple myeloma.
| Cat.No | ACP03256 | Target Name | CRBN |
|---|---|---|---|
| Target Synonyms | CRBN; AD-006; Protein cereblon | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 1-442aa |
| Mol Weight | 52.5 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q96SW2 |
|---|
Uniprot Id
Q96SW2
Target Species
Human
Target Name
CRBN
Target Full Name
Protein cereblon
Target Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.
Target Involvement
Mental retardation, autosomal recessive 2A (MRT2A)
Target Subcellular Location
Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
Target Protein Families
CRBN family
Target Tissue Specificity
Widely expressed. Highly expressed in brain.
Target Research Area
Neuroscience
Target Synonyms
CRBN; AD-006; Protein cereblon
Target Background
This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
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