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The preparation of the recombinant Human PARP12 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (1-701aa) of foreign DNA is fused to an expression vector and then introduced into e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant targeted protein is subjected to affinity purification. Its purity exceeds 90% as assessed by SDS-PAGE.
The preparation of the recombinant Human PARP12 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (1-701aa) of foreign DNA is fused to an expression vector and then introduced into e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant targeted protein is subjected to affinity purification. Its purity exceeds 90% as assessed by SDS-PAGE.
| Cat.No | ACP01177 | Target Name | PARP12 |
|---|---|---|---|
| Target Synonyms | (ADP-ribosyltransferase diphtheria toxin-like 12)(ARTD12)(Poly [ADP-ribose] polymerase 12)(PARP-12)(Zinc finger CCCH domain-containing protein 1) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-701aa |
| Mol Weight | 86.5 kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9H0J9 |
|---|
Uniprot Id
Q9H0J9
Target Species
Human
Target Name
PARP12
Target Full Name
Protein mono-ADP-ribosyltransferase PARP12
Target Function
Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.
Target Subcellular Location
Nucleus.
Target Research Area
Others
Target Synonyms
9930021O16; AA409132; AA536654; FLJ22693; HGNC:21919; MST109; MSTP109; OTTMUSP00000026964; PAR12_HUMAN; PARP 12; PARP-12; Parp12; Poly (ADP ribose) polymerase family member 12; Poly [ADP-ribose] polymerase 12; ZC3H1; ZC3HDC1; Zinc finger CCCH domain-containing protein 1; Zinc finger CCCH type domain containing 1
Target Background
Enables protein ADP-ribosylase activity. Involved in protein auto-ADP-ribosylation and protein mono-ADP-ribosylation. Predicted to be located in nucleus.
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