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The recombinant Human S100A7A protein production in Yeast cells involves the integration of a DNA fragment encoding the Human S100A7A protein (2-101aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the S100A7A protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human S100A7A protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human S100A7A protein reaches over 90%.
The recombinant Human S100A7A protein production in Yeast cells involves the integration of a DNA fragment encoding the Human S100A7A protein (2-101aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the S100A7A protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human S100A7A protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human S100A7A protein reaches over 90%.
| Cat.No | ACP02532 | Target Name | S100A7A |
|---|---|---|---|
| Target Synonyms | S100A7A; S100A15; S100A7L1; Protein S100-A7A; S100 calcium-binding protein A15; S100 calcium-binding protein A7-like 1; S100 calcium-binding protein A7A | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Expression Range | 2-101aa |
| Mol Weight | 13.2kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q86SG5 |
|---|
Uniprot Id
Q86SG5
Target Species
Human
Target Name
S100A7A
Target Full Name
Protein S100-A7A
Target Function
May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
Target Subcellular Location
Cytoplasm.
Target Protein Families
S-100 family
Target Tissue Specificity
Overexpressed in psoriasis.
Target Research Area
Cell Biology
Target Synonyms
S100A7A; S100A15; S100A7L1; Protein S100-A7A; S100 calcium-binding protein A15; S100 calcium-binding protein A7-like 1; S100 calcium-binding protein A7A
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