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The expression of recombinant human WNT5A protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 62-380aa of the human WNT5A protein. This N-terminal GST-tagged recombinant human WNT5A protein is also characterized by high purity, >85%. Under SDS-PAGE condition, this recombinant WNT5A protein migrated to the band of about 40 kDa molecular weight. It may have applications in WNT5A-mediated cancer research.WNT5B is considered to be a non-classical Wnt protein in the process of fat differentiation. Wnt protein family includes at least 19 members, which is a group of secretory glycoproteins rich in cysteine and participates in many biological processes such as embryonic development, cell proliferation and differentiation, and even tumor occurrence. Wnt5b is a member of the same family of proteins as Wnt5a. Evidence has accumulated that Wnt5b expression is involved in mouse chondrocyte maturation. Wnt5b were reported to be highly expressed in head and neck squamous cell carcinomas. Other studies also found highly expressed in breast cancer with high invasive activity. Of late, the role of Wnt5b overexpression in cancer aggressiveness has been identified. For example, in lung cancer and pancreatic cancer cells, upregulated Wnt5b has been observed, but the biochemical properties and cancer-associated activities of Wnt5b have not been well characterized.
The expression of recombinant human WNT5A protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 62-380aa of the human WNT5A protein. This N-terminal GST-tagged recombinant human WNT5A protein is also characterized by high purity, >85%. Under SDS-PAGE condition, this recombinant WNT5A protein migrated to the band of about 40 kDa molecular weight. It may have applications in WNT5A-mediated cancer research.WNT5B is considered to be a non-classical Wnt protein in the process of fat differentiation. Wnt protein family includes at least 19 members, which is a group of secretory glycoproteins rich in cysteine and participates in many biological processes such as embryonic development, cell proliferation and differentiation, and even tumor occurrence. Wnt5b is a member of the same family of proteins as Wnt5a. Evidence has accumulated that Wnt5b expression is involved in mouse chondrocyte maturation. Wnt5b were reported to be highly expressed in head and neck squamous cell carcinomas. Other studies also found highly expressed in breast cancer with high invasive activity. Of late, the role of Wnt5b overexpression in cancer aggressiveness has been identified. For example, in lung cancer and pancreatic cancer cells, upregulated Wnt5b has been observed, but the biochemical properties and cancer-associated activities of Wnt5b have not been well characterized.
| Cat.No | ACP02308 | Target Name | WNT5A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 62-380aa | Mol Weight | 39.8 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P41221 |
|---|
Uniprot Id
P41221
Target Species
Human
Target Name
WNT5A
Target Full Name
Protein Wnt-5a
Target Function
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Target Involvement
Robinow syndrome, autosomal dominant 1 (DRS1)
Target Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Target Protein Families
Wnt family
Target Tissue Specificity
Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected i
Target Research Area
Neuroscience, Cancer
Target Synonyms
hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt-5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt 5a; WNT 5A protein; WNT 5A protein precursor; WNT5A; WNT5A protein precursor; WNT5A_HUMAN
Target Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.
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