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The process of expressing the recombinant human F2 protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 44-622aa of the human F2 protein and N-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-tagged recombinant F2 protein was obtained. This recombinant F2 protein is characterized by high purity (>90%, SDS-PAGE). This F2 protein ran along the gel to the band of approximately 66-105 kDa molecular weight.F2 is a gene providing instruction of making a protein named prothrombin (also known as coagulation factor II) in human and belongs to peptidase S1 family. This protein can be cleaved into 4 chains, including activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. It is the precursor of thrombin, which acts as a serine protease cleaving bonds after Arg and Lys and converting soluble fibrinogen into insoluble strands of fibrin and activating factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
The process of expressing the recombinant human F2 protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 44-622aa of the human F2 protein and N-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-tagged recombinant F2 protein was obtained. This recombinant F2 protein is characterized by high purity (>90%, SDS-PAGE). This F2 protein ran along the gel to the band of approximately 66-105 kDa molecular weight.F2 is a gene providing instruction of making a protein named prothrombin (also known as coagulation factor II) in human and belongs to peptidase S1 family. This protein can be cleaved into 4 chains, including activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. It is the precursor of thrombin, which acts as a serine protease cleaving bonds after Arg and Lys and converting soluble fibrinogen into insoluble strands of fibrin and activating factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
| Cat.No | ACP04732 | Target Name | F2 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 44-622aa | Mol Weight | 67.3kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P00734 |
|---|
Uniprot Id
P00734
Target Species
Human
Target Name
F2
Target Full Name
Prothrombin
Target Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Target Involvement
Factor II deficiency (FA2D); Ischemic stroke (ISCHSTR); Thrombophilia due to thrombin defect (THPH1); Pregnancy loss, recurrent, 2 (RPRGL2)
Target Subcellular Location
Secreted, extracellular space.
Target Protein Families
Peptidase S1 family
Target Tissue Specificity
Expressed by the liver and secreted in plasma.
Target Research Area
Cardiovascular
Target Synonyms
coagulation factor II (thrombin); Coagulation factor II; F2; Factor II; Prepro coagulation factor II; Prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; THRB; THRB_HUMAN; Thrombin heavy chain
Target Background
This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure.
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