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The recombinant Human PDP2 protein expression in e.coli cells requires the insertion of a DNA fragment encoding the Human PDP2 protein (67-529aa) into a plasmid vector and the transferral of this vector into e.coli cells. The positive cells are screened, cultured, and then induced to express the PDP2 protein. The protein carries a N-terminal 6xHis tag. The cells are lysed to harvest the recombinant Human PDP2 protein, which is purified through affinity purification and then detected by SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of this recombinant Human PDP2 protein is over 90%.
The recombinant Human PDP2 protein expression in e.coli cells requires the insertion of a DNA fragment encoding the Human PDP2 protein (67-529aa) into a plasmid vector and the transferral of this vector into e.coli cells. The positive cells are screened, cultured, and then induced to express the PDP2 protein. The protein carries a N-terminal 6xHis tag. The cells are lysed to harvest the recombinant Human PDP2 protein, which is purified through affinity purification and then detected by SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of this recombinant Human PDP2 protein is over 90%.
| Cat.No | ACP04251 | Target Name | PDP2 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 67-529aa | Mol Weight | 56.4kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9P2J9 |
|---|
Uniprot Id
Q9P2J9
Target Species
Human
Target Name
PDP2
Target Full Name
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial
Target Function
Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Target Subcellular Location
Mitochondrion matrix.
Target Protein Families
PP2C family
Target Research Area
Metabolism
Target Synonyms
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2; KIAA1348 ; mitochondrial; PDP 2; Pdp2; PDP2_HUMAN; PDPC 2; PPM2B; PPM2C2; Protein phosphatase 2C magnesium dependent catalytic subunit 2; Protein phosphatase Mg2+/Mn2+ dependent 2B; Pyruvate dehydrogenase phosphatase 2; Pyruvate dehydrogenase phosphatase catalytic subunit 2; pyruvate dehydrogenase phosphatase isoenzyme 2 ; Pyruvate dehydrogenase phosphatase; catalytic subunit 2
Target Background
This gene is a mitochondrial protein that functions as a phosphatase and is involved in the enzymatic resetting of the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants encoding the same protein.
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