Recombinant Human RNA-binding protein FUS (FUS)

The generation of the recombinant Human FUS protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (1-526aa). After transfection, the cells are cultured to express the desired protein. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. The recombinant Human FUS protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 90%, as determined by SDS-PAGE.

ACP01320

Number

Cat.No	ACP01320	Target Name	FUS
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-526aa	Mol Weight	60.9 kDa
Protein Length	Full length	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01320

Target Name

FUS

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-526aa

Mol Weight

60.9 kDa

Protein Length

Full length

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P35637

Background Information

Uniprot Id

P35637
Target Species

Human
Target Name

FUS
Target Full Name

RNA-binding protein FUS
Target Function

DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis.
Target Involvement

Angiomatoid fibrous histiocytoma (AFH); Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia (ALS6); Tremor, hereditary essential 4 (ETM4)
Target Subcellular Location

Nucleus.
Target Protein Families

RRM TET family
Target Tissue Specificity

Ubiquitous.
Target Research Area

Cancer
Target Synonyms

75 kDa DNA pairing protein; 75 kDa DNA-pairing protein; ALS6; Amyotrophic lateral sclerosis 6; fus; FUS CHOP; Fus like protein; FUS_HUMAN; FUS1; Fused in sarcoma; Fusion (involved in t(12,16) in malignant liposarcoma); Fusion derived from t(12,16) malignant liposarcoma; Fusion gene in myxoid liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; hnRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA binding protein FUS; RNA-binding protein FUS; TLS; TLS CHOP; Translocated in liposarcoma; Translocated in liposarcoma protein
Target Background

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Recombinant Human RNA-binding protein FUS (FUS)

Specifications

Immunogen Information

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