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The expression region of this recombinant Human SKP2 covers amino acids 1-424. The theoretical molecular weight of the SKP2 protein is 63.8 kDa. The SKP2 protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the SKP2 encoding gene fragment was conducted, allowing for easier detection and purification of the SKP2 protein in subsequent stages.Human S-phase kinase-associated protein 2 (SKP2) is a key regulator of the cell cycle, specifically the G1 to S phase transition. SKP2 targets cell cycle inhibitors for ubiquitin-mediated degradation, promoting cell cycle progression. Studying SKP2 contributes to insights into cancer biology, cell cycle regulation, and potential therapeutic strategies targeting cell proliferation and differentiation. In cancer research, SKP2 is associated with tumor development and progression. In cell biology, it is crucial to understand cell cycle control. Additionally, SKP2 plays a role in stem cell maintenance.
The expression region of this recombinant Human SKP2 covers amino acids 1-424. The theoretical molecular weight of the SKP2 protein is 63.8 kDa. The SKP2 protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the SKP2 encoding gene fragment was conducted, allowing for easier detection and purification of the SKP2 protein in subsequent stages.Human S-phase kinase-associated protein 2 (SKP2) is a key regulator of the cell cycle, specifically the G1 to S phase transition. SKP2 targets cell cycle inhibitors for ubiquitin-mediated degradation, promoting cell cycle progression. Studying SKP2 contributes to insights into cancer biology, cell cycle regulation, and potential therapeutic strategies targeting cell proliferation and differentiation. In cancer research, SKP2 is associated with tumor development and progression. In cell biology, it is crucial to understand cell cycle control. Additionally, SKP2 plays a role in stem cell maintenance.
| Cat.No | ACP04626 | Target Name | SKP2 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-424aa | Mol Weight | 63.8kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q13309 |
|---|
Uniprot Id
Q13309
Target Species
Human
Target Name
SKP2
Target Full Name
S-phase kinase-associated protein 2
Target Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration.; Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Target Subcellular Location
Cytoplasm. Nucleus.
Target Research Area
Cancer
Target Synonyms
CDK2/Cyclin A associated protein p45; Cyclin A/CDK2 associated protein p45; Cyclin-A/CDK2-associated protein p45; F box protein Skp2; F box/LRR repeat protein 1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBL 1; FBL1; FBXL 1; FBXL1; FLB 1; FLB1; MGC1366; p45; p45skp2; S phase kinase associated protein 2 (p45); S phase kinase associated protein 2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 E3 ubiquitin protein ligase; SKP 2; Skp2; SKP2_HUMAN
Target Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.
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