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Insertion of the gene encoding the Human SALL4 protein (954-1053aa+11R) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Following expression, the recombinant Human SALL4 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human SALL4 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.
Insertion of the gene encoding the Human SALL4 protein (954-1053aa+11R) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Following expression, the recombinant Human SALL4 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human SALL4 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.
| Cat.No | ACP00009 | Target Name | SALL4 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Baculovirus |
| Expression Range | 954-1053aa+11R | Mol Weight | 16 |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9UJQ4 |
|---|
Uniprot Id
Q9UJQ4
Target Species
Human
Target Name
SALL4
Target Full Name
Sal-like protein 4
Target Function
Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
Target Involvement
Duane-radial ray syndrome (DRRS); Oculootoradial syndrome (OORS)
Target Subcellular Location
Cytoplasm. Nucleus.
Target Protein Families
Sal C2H2-type zinc-finger protein family
Target Tissue Specificity
Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML).
Target Research Area
Developmental Biology
Target Synonyms
AA407717; AL022809; AW536104; C330011P20Rik; C78083; C78563; dJ1112F19.1; DRRS; HSAL4; Sal like 4 (Drosophila); Sal like 4; Sal like Protein 4; Sal-like protein 4; Sall4; SALL4_HUMAN; Spalt like transcription factor 4; Tex20; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797
Target Background
This gene encodes a zinc finger transcription factor thought to play a role in the development of abducens motor neurons. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). Alternative splicing results in multiple transcript variants encoding different isoforms.
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