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This recombinant human serine/threonine-protein phosphatase 5 (PPP5C) is expressed in E. coli. The production process begins by introducing a gene fragment encoding the full-length mature human PPP5C protein (amino acids 2-499) into a suitable expression vector. The vector is designed to incorporate an N-terminal 10xHis-tag and a C-terminal Myc-tag for easy detection and purification of the recombinant protein. The recombinant construct is then transformed into E. coli cells, followed by the selection of transformed cells to ensure the presence of the recombinant plasmid. Subsequently, a large-scale culture is established, providing optimal conditions for protein expression. The expressed recombinant PPP5C protein is harvested from the cells and purified to achieve a high level of purity. SDS-PAGE analysis confirms the purity of the protein, which reaches up to 85%. When visualized on the gel, the recombinant Gpi protein migrates as a distinct band with an approximate molecular weight of 66 kDa.
This recombinant human serine/threonine-protein phosphatase 5 (PPP5C) is expressed in E. coli. The production process begins by introducing a gene fragment encoding the full-length mature human PPP5C protein (amino acids 2-499) into a suitable expression vector. The vector is designed to incorporate an N-terminal 10xHis-tag and a C-terminal Myc-tag for easy detection and purification of the recombinant protein. The recombinant construct is then transformed into E. coli cells, followed by the selection of transformed cells to ensure the presence of the recombinant plasmid. Subsequently, a large-scale culture is established, providing optimal conditions for protein expression. The expressed recombinant PPP5C protein is harvested from the cells and purified to achieve a high level of purity. SDS-PAGE analysis confirms the purity of the protein, which reaches up to 85%. When visualized on the gel, the recombinant Gpi protein migrates as a distinct band with an approximate molecular weight of 66 kDa.
| Cat.No | ACP01262 | Target Name | PPP5C |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-499aa | Mol Weight | 64.2 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P53041 |
|---|
Uniprot Id
P53041
Target Species
Human
Target Name
PPP5C
Target Full Name
Serine/threonine-protein phosphatase 5
Target Function
Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. Dephosphorylates and may play a role in the regulation of TAU/MAPT. Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2. Dephosphorylate FNIP1, disrupting interaction with HSP90AA1/Hsp90.
Target Subcellular Location
Nucleus. Cytoplasm. Cell membrane.
Target Protein Families
PPP phosphatase family, PP-5 (PP-T) subfamily
Target Tissue Specificity
Ubiquitous.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
FLJ36922; FLJ55954; OTTHUMP00000165834; PP-T; PP5; PPP5; PPP5_HUMAN; Ppp5c; PPT; Protein phosphatase 5, catalytic subunit; Protein phosphatase T; Serine/threonine protein phosphatase 5; Serine/threonine-protein phosphatase 5
Target Background
This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants.
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