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Constructing a plasmid encoding the Human SH3PXD2A protein (902-986aa) initiates the general approach for generating the recombinant Human SH3PXD2A protein. Transformation of the plasmid into e.coli cells obtains the plasmid-containing e.coli cells, which are cultured and induced for protein expression. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. Subsequently, the protein is purified through affinity purification, and SDS-PAGE analysis is undertaken to confirm the presence and assess the purity of the protein. The protein's purity exceeds 90%.
Constructing a plasmid encoding the Human SH3PXD2A protein (902-986aa) initiates the general approach for generating the recombinant Human SH3PXD2A protein. Transformation of the plasmid into e.coli cells obtains the plasmid-containing e.coli cells, which are cultured and induced for protein expression. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. Subsequently, the protein is purified through affinity purification, and SDS-PAGE analysis is undertaken to confirm the presence and assess the purity of the protein. The protein’s purity exceeds 90%.
| Cat.No | ACP02372 | Target Name | SH3PXD2A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 902-986aa | Mol Weight | 14.0 kDa |
| Protein Length | Partial | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q5TCZ1 |
|---|
Uniprot Id
Q5TCZ1
Target Species
Human
Target Name
SH3PXD2A
Target Full Name
SH3 and PX domain-containing protein 2A
Target Function
Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.
Target Subcellular Location
Cytoplasm. Cell projection, podosome. Note=Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
Target Protein Families
SH3PXD2 family
Target Tissue Specificity
Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.
Target Research Area
Others
Target Synonyms
Adapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains
Target Background
Predicted to enable superoxide-generating NADPH oxidase activator activity. Involved in osteoclast fusion and superoxide metabolic process. Located in podosome.
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