Recombinant Human SUMO-activating enzyme subunit 1 (SAE1)

Amino acids 1-346 constitute the expression domain of recombinant Human SAE1. This SAE1 protein is theoretically predicted to have a molecular weight of 65.4 kDa. This protein is generated in a e.coli-based system. The N-terminal GST tag was fused into the coding gene segment of SAE1, making it easier to detect and purify the SAE1 recombinant protein in the later stages of expression and purification.The human SUMO-activating enzyme subunit 1 (SAE1) is a critical component in the SUMOylation pathway, an essential post-translational modification process. SAE1 forms a heterodimer with SAE2 to activate the Small Ubiquitin-like Modifier (SUMO) proteins. This activation initiates the attachment of SUMO to target proteins, influencing their cellular localization, interactions, and functions. SAE1's main function lies in catalyzing the adenylation and subsequent conjugation of SUMO to target proteins. Research areas involving SAE1 encompass investigations into cellular processes like DNA repair, transcriptional regulation, and response to cellular stress. Understanding SAE1's role in SUMOylation provides insights into the complex regulatory mechanisms governing diverse cellular functions and potential implications in diseases like cancer and neurodegeneration.