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The intact gene sequence of the human three-prime repair exonuclease 1 (TREX1) is expressed in the baculovirus. The resulting protein is fused with the MBP-tag at the N-terminus and the 6xHis-tag at the C-terminus, ultimately leading to the production of the recombinant human TREX1 (CSB-BP865133HU). Its purity is greater than 85% determined by SDS-PAGE. It migrated to the molecular weight band of approximately 65 kDa. The target protein TREX1 plays an important role in genomic DNA degradation, cell death processes, and gap-filling during DNA repair or proofreading during lagging-strand DNA synthesis.
The intact gene sequence of the human three-prime repair exonuclease 1 (TREX1) is expressed in the baculovirus. The resulting protein is fused with the MBP-tag at the N-terminus and the 6xHis-tag at the C-terminus, ultimately leading to the production of the recombinant human TREX1 (CSB-BP865133HU). Its purity is greater than 85% determined by SDS-PAGE. It migrated to the molecular weight band of approximately 65 kDa. The target protein TREX1 plays an important role in genomic DNA degradation, cell death processes, and gap-filling during DNA repair or proofreading during lagging-strand DNA synthesis.
| Cat.No | ACP03209 | Target Name | TREX1 |
|---|---|---|---|
| Target Synonyms | TREX1; Three-prime repair exonuclease 1; EC 3.1.11.2; 3'-5' exonuclease TREX1; Deoxyribonuclease III; DNase III | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 1-369aa |
| Mol Weight | 82.9 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9NSU2 |
|---|
Uniprot Id
Q9NSU2
Target Species
Human
Target Name
TREX1
Target Full Name
Three-prime repair exonuclease 1
Target Function
Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Target Involvement
Aicardi-Goutieres syndrome 1 (AGS1); Systemic lupus erythematosus (SLE); Chilblain lupus 1 (CHBL1); Vasculopathy, retinal, with cerebral leukodystrophy (RVCL)
Target Subcellular Location
Nucleus. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein.
Target Protein Families
Exonuclease superfamily, TREX family
Target Tissue Specificity
Detected in thymus, spleen, liver, brain, heart, small intestine and colon.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
TREX1; Three-prime repair exonuclease 1; EC 3.1.11.2; 3'-5' exonuclease TREX1; Deoxyribonuclease III; DNase III
Target Background
This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants.
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