Recombinant Human Transcriptional coactivator YAP1 (YAP1)

The fusion tag N-terminal 6xHis-B2M tag gene was added to the gene sequence corresponding to the E.coli of the human YAP1 protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transformed into the E.coli for expression. Following purification, the product is the recombinant human YAP1 protein carrying N-terminal 6xHis-B2M tag. The SDS-PAGE assessed the purity of this recombinant YAP1 protein up to 85%. It had an apparent molecular weight of approximately 78 kDa. This recombinant YAP1 protein may be used in YAP1-mediated cancer research.YAP1 is a gene encoding a protein named transcriptional coactivator YAP1 (abbreviated YAP1) in human and belongs to YAP1 family. This protein acts as a transcriptional regulator by activating the transcription of genes involved in cell proliferation and suppressing apoptotic genes. It is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Reported diseases associated with YAP1 include Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/Palate, And/Or Mental Retardation and Coloboma Of Macula.

ACP03105

Number

Cat.No	ACP03105	Target Name	YAP1
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-504aa	Mol Weight	68.5 kDa
Protein Length	Full length	Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP03105

Target Name

YAP1

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-504aa

Mol Weight

68.5 kDa

Protein Length

Full length

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P46937

Background Information

Uniprot Id

P46937
Target Species

Human
Target Name

YAP1
Target Full Name

Transcriptional coactivator YAP1
Target Function

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation.; Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).; Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Target Involvement

Coloboma, ocular, with or without hearing impairment, cleft lip/palate, and/or mental retardation (COB1)
Target Subcellular Location

Cytoplasm. Nucleus.
Target Protein Families

YAP1 family
Target Tissue Specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Target Research Area

Cancer
Target Synonyms

65 kDa Yes associated protein; 65 kDa Yes-associated protein; COB1; YAp 1; YAP 65; YAP; YAP-1; YAP1; YAP1_HUMAN; YAP2; YAP65; yes -associated protein delta; Yes associated protein 1 65kDa; Yes associated protein 1; Yes associated protein 2; yes associated protein beta; YKI; Yorkie homolog
Target Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Recombinant Human Transcriptional coactivator YAP1 (YAP1)

Specifications

Immunogen Information

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