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In the E. coli expression system, the production process of the recombinant human Tyrosine-protein phosphatase non-receptor type 11 (PTPN11) (T253M, Q257L) tagged with an N-terminal 6xHis-tag involves several steps. The gene fragment encoding the full-length mature human PTPN11 protein (2-593aa), including the T253M and Q257L mutations, is cloned into a suitable expression vector. The vector is designed to incorporate an N-terminal 6xHis-tag downstream of the gene fragment, allowing for convenient purification and detection of the recombinant protein. The constructed recombinant plasmid is transformed into E. coli host cells and transformed cells are selected and cultured in a growth medium optimized for recombinant protein expression. Following an appropriate incubation period, the cells are harvested and lysed to release the intracellular contents, including the expressed recombinant PTPN11 protein. The purity of the purified recombinant PTPN11 protein is assessed using SDS-PAGE analysis, measuring up to 90%.
In the E. coli expression system, the production process of the recombinant human Tyrosine-protein phosphatase non-receptor type 11 (PTPN11) (T253M, Q257L) tagged with an N-terminal 6xHis-tag involves several steps. The gene fragment encoding the full-length mature human PTPN11 protein (2-593aa), including the T253M and Q257L mutations, is cloned into a suitable expression vector. The vector is designed to incorporate an N-terminal 6xHis-tag downstream of the gene fragment, allowing for convenient purification and detection of the recombinant protein. The constructed recombinant plasmid is transformed into E. coli host cells and transformed cells are selected and cultured in a growth medium optimized for recombinant protein expression. Following an appropriate incubation period, the cells are harvested and lysed to release the intracellular contents, including the expressed recombinant PTPN11 protein. The purity of the purified recombinant PTPN11 protein is assessed using SDS-PAGE analysis, measuring up to 90%.
| Cat.No | ACP01189 | Target Name | PTPN11 |
|---|---|---|---|
| Target Synonyms | (Protein-tyrosine phosphatase 1D)(PTP-1D)(Protein-tyrosine phosphatase 2C)(PTP-2C)(SH-PTP2)(SHP-2)(Shp2)(SH-PTP3) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-593aa(T253M, Q257L) |
| Mol Weight | 73.9 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q06124 |
|---|
Uniprot Id
Q06124
Target Species
Human
Target Name
PTPN11
Target Full Name
Tyrosine-protein phosphatase non-receptor type 11
Target Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification.
Target Involvement
LEOPARD syndrome 1 (LPRD1); Noonan syndrome 1 (NS1); Leukemia, juvenile myelomonocytic (JMML); Metachondromatosis (MC)
Target Subcellular Location
Cytoplasm. Nucleus.
Target Protein Families
Protein-tyrosine phosphatase family, Non-receptor class 2 subfamily
Target Tissue Specificity
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Target Research Area
Neuroscience
Target Synonyms
(Protein-tyrosine phosphatase 1D)(PTP-1D)(Protein-tyrosine phosphatase 2C)(PTP-2C)(SH-PTP2)(SHP-2)(Shp2)(SH-PTP3)
Target Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
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