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The formation of the recombinant plasmid by incorporating the gene encoding the Human PTPN5 protein (300-555aa) into a plasmid vector initiates the generation of the recombinant Human PTPN5 protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human PTPN5 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human PTPN5 protein, revealing a purity level exceeding 90%.
The formation of the recombinant plasmid by incorporating the gene encoding the Human PTPN5 protein (300-555aa) into a plasmid vector initiates the generation of the recombinant Human PTPN5 protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human PTPN5 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human PTPN5 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP04423 | Target Name | PTPN5 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 300-555aa | Mol Weight | 45.5kDa |
| Protein Length | Partial | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P54829 |
|---|
Uniprot Id
P54829
Target Species
Human
Target Name
PTPN5
Target Full Name
Tyrosine-protein phosphatase non-receptor type 5
Target Function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Target Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Target Protein Families
Protein-tyrosine phosphatase family, Non-receptor class subfamily
Target Synonyms
FLJ14427; Neural specific protein tyrosine phosphatase; Neural-specific protein-tyrosine phosphatase; Protein tyrosine phosphatase non receptor type 5 (striatum enriched); Protein tyrosine phosphatase non receptor type 5; Protein tyrosine phosphatase striatum enriched; PTN5; PTN5_HUMAN; PTP STEP; PTPN 5; Ptpn5; PTPSTEP; STEP; Striatum-enriched protein-tyrosine phosphatase; Tyrosine protein phosphatase non receptor type 5; Tyrosine-protein phosphatase non-receptor type 5
Target Background
Enables phosphotyrosine residue binding activity. Predicted to be involved in peptidyl-tyrosine dephosphorylation. Predicted to act upstream of or within protein dephosphorylation. Predicted to be located in nucleoplasm. Predicted to be integral component of membrane. Biomarker of Alzheimer's disease.
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