Recombinant Human Vacuolar protein sorting-associated protein 4A (VPS4A)

Uniprot Id

Q9UN37

Target Species

Human

Target Name

VPS4A

Target Full Name

Vacuolar protein sorting-associated protein 4A

Target Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.; (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

Target Subcellular Location

Late endosome membrane; Peripheral membrane protein. Midbody.

Target Protein Families

AAA ATPase family

Target Tissue Specificity

Ubiquitously expressed.

Target Research Area

Others

Target Synonyms

hVPS4; Protein SKD2; SKD1; SKD1 homolog; SKD1A; SKD2; Vacuolar protein sorting 4 homolog A; Vacuolar protein sorting 4, yeast, homolog of, A; Vacuolar protein sorting factor 4A; Vacuolar protein sorting-associated protein 4A; vacuolar sorting protein 4; VPS4; VPS4-1; Vps4a; VPS4A_HUMAN

Target Background

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.