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Recombination of a plasmid encoding the Human VSNL1 protein (1-191aa) is the first step during the production the recombinant Human VSNL1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is used to isolate and purify the recombinant Human VSNL1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human VSNL1 protein. Its purity exceeds 90%.
Recombination of a plasmid encoding the Human VSNL1 protein (1-191aa) is the first step during the production the recombinant Human VSNL1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is used to isolate and purify the recombinant Human VSNL1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human VSNL1 protein. Its purity exceeds 90%.
| Cat.No | ACP03542 | Target Name | VSNL1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-191aa | Mol Weight | 49.0kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P62760 |
|---|
Uniprot Id
P62760
Target Species
Human
Target Name
VSNL1
Target Full Name
Visinin-like protein 1
Target Function
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
Target Protein Families
Recoverin family
Target Tissue Specificity
Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level).
Target Research Area
Signal Transduction
Target Synonyms
21 kDa CABP; Hippocalcin like protein 3; Hippocalcin-like protein 3; HLP 3; HLP3; HPCAL 3; HPCAL3; HUVISL1; Neural visinin-like protein 1; Neural visinin-like type 1 protein; Neurocalcin alpha; NVL 1; NVL1; Nvp1; OZ1; Ratnvp1; VILIP; VILIP-1; Visinin; Visinin like 1; Visinin like protein 1; Visinin-like protein 1; VISL 1; VISL1; VISL1_HUMAN; VLP-1; Vnsl1; Vsnl1
Target Background
This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
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